PCR detects fungal DNA in keratitis

Article

Polymerase chain reaction (PCR) could be used to diagnose fungal keratitis.

Polymerase chain reaction (PCR) could be used to diagnose fungal keratitis, claims an investigation in the journal Eye.

A team led by Dr N. Tananuvat, Department of Ophthalmology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, conducted a prospective nonrandomized study on 30 eyes of 30 patients with presumed fungal keratitis.

Each patient underwent corneal scraping and fungal-specific primers were used to analyse the specimens. PCR products were cloned and sequenced and compared to conventional microbial work-up.

PCR demonstrated positivity in 93.3%, culture in 40% and potassium hydroxide in 20% of the total patients included in the study. Out of the 28 PCR-positive patients, 12 were culture-positive and 16 were culture-negative.

There were no false-negative results in the culture-positive specimens recorded by the PCR, suggesting it is able to accurately detect fungal DNA in a high percentage of culture-negative cases. The procedure could be helpful, in addition to smear and culture in the efficient diagnosis of fungal keratitis.

Please click here to read the abstract.

Related Videos
ARVO 2024: Andrew D. Pucker, OD, PhD on measuring meibomian gland morphology with increased accuracy
 Allen Ho, MD, presented a paper on the 12 month results of a mutation agnostic optogenetic programme for patients with severe vision loss from retinitis pigmentosa
Noel Brennan, MScOptom, PhD, a clinical research fellow at Johnson and Johnson
ARVO 2024: President-elect SriniVas Sadda, MD, speaks with David Hutton of Ophthalmology Times
Elias Kahan, MD, a clinical research fellow and incoming PGY1 resident at NYU
Neda Gioia, OD, sat down to discuss a poster from this year's ARVO meeting held in Seattle, Washington
Eric Donnenfeld, MD, a corneal, cataract and refractive surgeon at Ophthalmic Consultants of Connecticut, discusses his ARVO presentation with Ophthalmology Times
John D Sheppard, MD, MSc, FACs, speaks with David Hutton of Ophthalmology Times
Paul Kayne, PhD, on assessing melanocortin receptors in the ocular space
Osamah Saeedi, MD, MS, at ARVO 2024
© 2024 MJH Life Sciences

All rights reserved.